Considerations To Know About cytometers
Considerations To Know About cytometers
Blog Article
Data-enriched characterization of mobile populations. Morphological details from illustrations or photos can increase into the richness of flow cytometry knowledge. One example is, the determine exhibits an normally typical apoptosis assay using Annexin V and PI, introducing cell imaging to characterize cells in each inhabitants to expose morphologically unique attributes. These insights couldn't are obtained from multiplex staining alone.
As samples are acquired on the Attune CytPix Flow Cytometer, the higher-velocity brightfield digital camera captures and outlets images of detected functions in a charge around 6,000 photographs for every 2nd, based on the flow fee and impression size. For greater versatility, Attune Cytometric Software package helps you to adjust the image seize frequency as required and select a selected gate for impression seize, if essential.
Merge acoustic focusing technologies with high-parameter Examination for versatile spectral unmixing and conventional workflows.
The award-profitable Invitrogen Attune CytPix Flow Cytometer brings together acoustic concentrating having a high-pace brightfield camera for simultaneous higher throughput flow cytometry and substantial resolution brightfield imaging.
"An image is not rather value 1000 dots, but it definitely helps to see images. It is excellent to discover photos that correlate with staining. One example is, AnnexinV-optimistic cells which have been morphologically different than balanced cells, or CD14+ monocytes which have been much larger and a lot more textured than CD3+/CD4+/CD14- lymphocytes.
Here, a seasoned person has gated singlets confidently. Just after analyzing the manual singlet gate, the CytPix impression-derived parameter ParticleCount reveals this gate includes more than 4% aggregates.
Optimizing gating tactic. Even robust guide singlet gating is mistake-susceptible and stays a subjective conclusion place in Just about all flow cytometry assays. Imaging can be employed to substantiate and modify gates to include only solitary cells of curiosity.
Our researchers will design and style panels for you as persistently you may need—cost-free, no merchandise buy necessary.
Early indications of this contamination experienced at first been dismissed as debris. Backgating to find new populations. Backgating imaged cells to the Attune CytPix Flow Cytometer also helps you to use morphological functions to find out fascinating subpopulations that might not be obvious from flow cytometry info alone.
Most likely most of all, these occasions have cells of Obviously diverse phenotypes which could lead to incorrect conclusions about double constructive gatherings (especially in exceptional populations).
AI-pushed automatic picture analysis With all the Attune Cytometric Program interprets event attributes into unique morphology parameters that could be coupled with regular fluorescence and scatter parameters to expose highly reproducible population changes and unique subsets.
Application Take note: Automatic image Examination cuts down consumer-to-person variability check here in flow cytometry gating strategies
Leverage the strength of State-of-the-art technological innovation and innovation to speed up your discoveries. Rely on in the Attune Flow Cytometer family to deliver the reliability, speed, and precision that established your function aside.
Adaptable payment program from Agilent Financial Methods help you get the important instrumentation you require for quick undertaking, or to mature your lab.
At the center of every Attune Flow Cytometer lies our revolutionary acoustic focusing technology, which makes use of audio waves to meticulously align cells in the flow mobile. This modern method surpasses common hydrodynamic approaches, causing a lot quicker processing speeds, Extraordinary details quality, and greater sample throughput.
Because the mobile line experienced Earlier been analyzed about the Attune CytPix Flow Cytometer, the researchers went back again to the images and were being capable of doc the microbial an infection a minimum of five days previously.
Agilent Price Assure assures you not less than a decade of instrument use from your date you buy. In any other case, We're going to credit score you With all the residual value of the program toward an upgraded design.
What appeared to be a homogeneous inhabitants is definitely more varied—an insight that needs to be regarded as when interpreting outcomes.